PSG4, CFI, CHI3L1, BDKRB2, GUCY1A2, SFRP4, IL33, and PADI2 were upregulated in the control group but downregulated in the PCOS group in both datasets, and PITX1, ISG15, NEURL1, OAS2, PRSS3, PI16, L3MBTL4, and MX2 were downregulated in the control group but upregulated in the PCOS group in both datasets. The gene discussed is ISG15; the disease is polycystic ovary syndrome.