Further, functionally, five proteins upregulated in DMD were associated with muscle contraction (GO Term enrichment analysis p-value 1.5 × 10−2), including Myosin light polypeptide 6, Tropomyosin alpha-1 chain, Myoferlin, Caldesmon, and Tropomyosin alpha-4 chain. The gene discussed is TPM1; the disease is Duchenne muscular dystrophy.