To validate our findings, future studies should validate co-expressed genes such as Dnajb11, Edem3, Rnf185, Hspa1l/Hspa2, Ambra1, Mfn2, Usp30, Drp1, Camk2d, Kcnh2 at the protein level using Western blot (WB) in 16-week-old HSALR mice without severe DM1 symptoms. The gene discussed is HSPA1L; the disease is myotonic dystrophy type 1.