Building on our preliminary findings showing that this strain produces extracellular pigments with potential cosmetic relevance [19], the objectives of the present study were to: (i) optimize pigment production through factorial experimental design; (ii) characterize the pigment-rich fraction by FTIR and LC–MS; and (iii) assess its bioactivities, including anti-tyrosinase, anti-inflammatory, and anti-acne properties. This evidence concerns the gene TYR and acne.