TP53 and Familial prostate cancer: In this regard, previous studies reported an increased expression of p53 and caspase-3 in HeLa cells at concentrations of GB between 20 mg/mL and 100 mg/mL [7], and an increased expression level of caspase-3 was associated with cellular death in the human prostate cancer cell line DU-145 incubated with 50 mg/mL of GB [6].