The main aim of this study is to assess the presence of MSX1, RYK, NFκB p65, and CCL4 proteins by immunohistochemistry (IHC) and MSX2, RYK, and PTX3 gene-signal-containing cells by using chromogenic in situ hybridization (CISH) in cleft lip and control tissue by using the semiquantitative counting method. Here, NFKB1 is linked to cleft lip.