CHAT and neuroblastoma: In addition to ChAT, we performed flow cytometric analyses to assess the expression and localization of selected components of the cholinergic machinery, namely the ACh-hydrolyzing enzymes AChE and BChE, as well as the α7 nicotinic AChR (α7 AChR) and the M1-subtype of muscarinic AChR (M1 AChR) in SH-SY5Y neuroblastoma cells.