ACHE and Cognitive impairment: A statistical evaluation using two-way ANOVA indicated a significant influence of the treatment on AChE activity [F(1, 40) = 8.138, p = 0.0068], as shown in Figure 8A. Acute administration of SCOP before euthanasia led to a substantial increase in AChE activity in the zebrafish brain when compared to the control cohort (p < 0.0001), suggesting cholinergic dysfunction associated with cognitive impairment.