To evaluate differences in viral infection efficiency between pyroptotic and apoptotic/necroptotic cell death, we conducted immunofluorescence staining using antibodies against MPXV (viral infection marker) alongside ASC (pyroptosis marker), cleaved CASP3 (apoptosis marker), or phosphorylated MLKL (pMLKL) (necroptosis marker). This evidence concerns the gene CASP3 and viral infectious disease.