ACTA2 and cancer: Gene ontology enrichment results for these gene lists coincide with the idea that PC1 represents the EMT axis: PC1 positive genes have functions related to cell adhesion (tenascin C, JAG1, ACTA2), extracellular matrix organization (APP, LAMA2, FGF2, BMP4) and positive regulation of cell migration, consistent with a mesenchymal phenotype, while PC1 negative genes show GO terms related to epithelial maturation (Figure 1d) Overall, our analyses show that the genome organization is not only able to distinguish different subtypes of cancers but also exhibits changes along the EMT.