ASCL1 and neoplasm: demonstrated via multiplex immunofluorescence that within the same tumor, NE subpopulations (e.g., SCLC-A) specifically overexpress proteins such as INSM1 and ASCL1, while adjacent non-NE subpopulations (e.g., SCLC-P) are enriched with POU2F3 and Vimentin.