Zeng et al. (65) demonstrated that platycodin D upregulated the expression of cytochrome C by regulating Bcl-2 in gallbladder cancer, further validating the endogenous cell apoptosis mechanism induced by platycodin D. In addition, the anti-apoptosis mechanism of platycodin D has been confirmed in many cancers, such as breast cancer (50), colon cancer (52), gallbladder cancer (66), prostate cancer (67) and non-small cell lung cancer (68). This evidence concerns the gene CYCS and gallbladder cancer.