The PCOS group showed significantly higher insulin (mean difference: 5.2 μIU/mL, p = 0.003), HOMA-IR (p = 0.004), LH (p = 0.002), AMH (p < 0.001), and chloride (p < 0.001), with lower Na:Cl ratio (p < 0.001) and QUICKI (p = 0.005) compared to controls. This evidence concerns the gene INS and polycystic ovary syndrome.