EdU staining (Fig. 3F and G) and CCK8 analysis (Fig. 3H) revealed that in NR6A1-siRNA-treated cells, the addition of MHY1485 restored the proliferative phenotype of A549 cells to a certain extent, suggesting that NR6A1 promotes tumor cell glycolysis and proliferation via mTOR signaling. The gene discussed is MTOR; the disease is neoplasm.