To explore the impact of food intake reduction on the obesity protection phenotype, we performed a two-week pair-feeding experiment, and as shown in Fig. 7d, during the intervention, control mice maintained body mass similar to Fezf1-Bdnf knockout, whereas, as early as two weeks after retuning to ad libitum feeding, the body mass was significantly increased. The gene discussed is FEZF1; the disease is obesity due to melanocortin 4 receptor deficiency.