BDNF and obesity due to melanocortin 4 receptor deficiency: Inhibition of Fezf1 Bdnf protects against diet-induced obesity in female mice. Male and female Fezf1-cre/EGFP-L10a∆Bdnf (Fezf1-Bdnf knockout) mice were treated for three consecutive days with trimethoprim for inducing the conditional deletion of Bdfn specifically in Fezf1 neurons (Fig. 6a).