Inhibition of Fezf1 Bdnf protects against diet-induced obesity in female mice. Male and female Fezf1-cre/EGFP-L10a∆Bdnf (Fezf1-Bdnf knockout) mice were treated for three consecutive days with trimethoprim for inducing the conditional deletion of Bdfn specifically in Fezf1 neurons (Fig. 6a). Here, FEZF1 is linked to obesity due to melanocortin 4 receptor deficiency.