Subsequently, we conducted ITLN1 overexpression in SK-Hep1 and MHCC-97H cells, as well as ITLN1 knockdown in Huh7 and PLC/PRF/5 cells to investigate the role of ITLN1 in HCC (Figs. 2A and S2A). The gene discussed is ITLN1; the disease is hepatocellular carcinoma.