With the rapid development of sequencing technology, comprehensive bioinformatics analysis based on high-throughput sequencing has opened up an important way for studying the mechanisms of disease occurrence and developing new therapeutic strategies.6, 7, 8 Sun et al. used bioinformatics methods to screen diagnostic genes and revealed that knockdown of ISG20 distinctly suppressed the expression of the expressions of fibrotic proteins (α-SMA and fibronectin), indicating that ISG20 promotes the progression of renal fibrosis.9 Here, ACTA1 is linked to renal fibrosis.