From this experiment, tumor growth data, infiltrated CAR-T cells measured by multiphoton microscopy (number of cells per volume), immunofluorescence staining (number of cells per volume), and flow cytometry (percentage of CAR-T cells normalize to total T cells) as well as measurements of CD8+ T cells and FOXP3+ CD4+ T cells (Tregs) by flow cytometry (percentage of T cells over total T cells) (36) were employed for validation. The gene discussed is CD4; the disease is neoplasm.