Dissociated tumor cells were depleted for Cd45, which marks hematopoietic cells, sorted with fluorescently labeled F7-Ab to isolate hFzd7Hi and hFzd7Lo cell populations (Fig. 1B and SI Appendix, Fig. S3A) and then transplanted orthotopically into the 4th mammary fat pad of syngeneic mice. Here, PTPRC is linked to neoplasm.