In order to phenotypically investigate their role in AMR, 13 newly identified genes were selected for performing experiments with selected knockout among the 52 genes that satisfied the criteria described in the Methods - In Vitro Validation Through Antibiotic Susceptibility Testing section (see also Table S1): uhpB (JW3643-KC), mdaB (JW2996-KC), yieF (JW3691-KC), pitB (JW2955-KC), rplA (JW3947-KC), uvrB (JW0762-KC), rpmG (JW3611-KC), rpsF (JW4158-KC), nemA (JW1642-KC), ompC (JW2203-KC), ompT (JW0554-KC), yeaD (JW1769-KC) and yeaE (JW1770-KC). This evidence concerns the gene CALCA and keratoconus.