Functional validation confirmed TRIM33's role: mass spectrometric analysis of ASS1‐bound proteins detected TRIM33 peptides (Figure 5M), Co‐IP demonstrated their physical interaction in CRC cells (Figure 5N) and the binding energy −2.4 kcal mol−1 was calculated by molecular docking of TRIM33 and ASS1 based on AlphaFold3 software (Figure 5O). This evidence concerns the gene TRIM33 and colorectal carcinoma.