Increased NT and NF are well-established but non-specific soft markers, commonly linked to aneuploidies like Down syndrome (Methods In Medicine CAM, 2023) and Turner syndrome (Gravholt et al., 2017; Yana et al., 2024), but also frequently reported in numerous monogenic disorders such as Noonan syndrome (Tangshewinsirikul et al., 2024), Kabuki syndrome (Sun et al., 2023), and CHARGE syndrome (Hsu et al., 2014). Here, NFASC is linked to Down syndrome.