We used mice on a mixed C57Bl/6JxSv129 background, as on this background Pten+/− mice develop PHTS-relevant phenotypes, with an overlapping tumour spectrum including hyperplasia/tumours of the thyroid, endometrium, lymphoid tissue, small intestine and adenomyoepithelioma, and malignant mammary tumours in the females (Tibarewal et al., 2022). This evidence concerns the gene PTEN and PTEN hamartoma tumor syndrome.