PIK3CA and neoplasm: RNA‐based transcriptomics technologies, such as bulk RNA‐seq and scRNA‐seq (Figure 4A), analyze gene expression and transcriptional regulatory features to assess whether organoids reflect the functional state of the target tissue at the molecular level.[227] RNA‐seq can reveal important gene mutations and expression patterns in organoid disease models, evaluating whether the activation of cancer‐related signaling pathways (e.g., Wnt, PI3K‐Akt) aligns with the primary tumor.[228, 229] Wang et al.