SPOP and colorectal carcinoma: By LC‒MS, we identified the O-GlcNAcylation modification sites S58 and S59 of SPOP in CRC, and the O-GlcNAcylation modification of SPOP can affect the binding of SPOP to β-catenin and change the ubiquitination level of β-catenin, these findings underscore the pivotal role of O-GlcNAcylation in modulating the functional activity of SPOP within the context of CRC.