Within the primary LUAD tumor microenvironment, as delineated in the single-cell map (Fig. 3A), significant upregulation of MZT2B expression was observed within malignant epithelial cells, with expression levels increasing concurrently with disease progression and exhibiting elevated expression in both early and advanced disease stages compared to normal tissue (Fig. 3B). The gene discussed is MZT2B; the disease is neoplasm.