PGK1 and neoplasm: Consistent with our in vitro findings, tumors with endogenous PGK1 depletion and reconstituted expression of rPGK1 S256A exhibited significantly slower growth, reduced tumor volume (Figs. 6B, C), and tumor weight (Fig. 6D), as well as lower Ki67 expression (Fig. 6E) and higher γ-H2AX expression (Fig. 6E) compared to WT rPGK1-expressing tumors after IR.