To further clarify the role of TMEM27 on ferroptosis and radiosensitivity in HNSCC, we firstly detected the expression of TMEM27 at different time points post 4 Gy irradiation in HNSCC cells, which showed that the expression of TMEM27 was decreased at 12 h and 24 h following 4 Gy irradiation in HN8 cells (Fig. S6A). The gene discussed is CLTRN; the disease is head and neck squamous cell carcinoma.