Itraconazole, an antifungal agent, suppresses HCC cell growth and induces apoptosis via multiple mechanisms, including inhibition of the Hedgehog, Wnt/β-catenin, and AKT/mTOR/ribosomal protein S6 kinase (S6K) signaling pathways, as well as by inducing ROS production and activating death receptor signaling 89. This evidence concerns the gene AKT1 and hepatocellular carcinoma.