DUSP6 and lung cancer: To investigate the potential of OCT4 to transactivate the DUSP6 promoter, we cloned the human DUSP6 promoter region (~2 kb) upstream of the transcription start site into a dual-luciferase reporter vector pFRL2 and co-transfected this reporter plasmid with an OCT4 expression plasmid or a control vector into A549, H1299 and CL1-5 lung cancer cell lines.