In the dataset GSE161529 we obtained 20 cell clusters by filtering the data, downscaling the analysis, and then clustering the cells using the UMAP method (Fig. 6D), and further manually annotated the resulting cell clusters, which finally resulted in 9 cell clusters of Epithelial cell, Cancer cell, fibroblast, B cell, Myeloid, Endothelial cell, CD4+ T cell, CD8+ T cell, Plasma blasts (Fig. 6E). The gene discussed is CD8A; the disease is cancer.