In RAW264.7, co-localization analysis of DAPI-stained nuclei (blue) and FITC-labeled LC3 (green) revealed that BCG infection (MOI = 10, 12 h) significantly increased LC3-positive puncta compared to uninfected controls (p<0.005), whereas HBHA treatment reduced puncta density substantially versus infection alone (Figures 5A, B). The gene discussed is MAP1LC3A; the disease is infection.