To evaluate the antitumor efficacy of CAR-T cells targeting the tumor-associated ganglioside GM3(Neu5Gc), we first generated a stable GM3(Neu5Gc)-expressing SKOV3 ovarian cancer cell line (SKOV3-CMAH) via lentiviral transduction of the murine CMAH gene, which reconstitutes the biosynthetic pathway for Neu5Gc. Here, CMAHP is linked to ovarian carcinoma.