Interestingly, while 14F7-CD28 and 14F7hT-CD28 CAR-T cells exhibited comparable in vitro function, including antigen specificity, cytokine secretion, and short-term cytotoxicity, the 14F7hT-CD28 CAR-T cells exhibited markedly enhanced in vivo expansion, persistence, and tumor control. This evidence concerns the gene CD28 and neoplasm.