The GSEA of MPO, CCL3 and TLR8 revealed that these 3 prognostic genes were closely associated with the “chemokine signaling pathway, we conducted GSEA on MPO, CCL3, and TLR8, revealing that these 3 prognostic genes are closely linked to the “chemokine signaling pathway.” This discovery holds significant importance in AML research, as it elucidates potential mechanisms through which these genes may contribute to AML progression. This evidence concerns the gene CCL3 and acute myeloid leukemia.