This study, however, does not address the types of solid foods introduced, the overlap between breastfeeding and solid feeding, or potential microbial functional changes, such as mammalian target of rapamycin (mTOR), AMP-activated protein kinase (AMPK), and the insulin signaling pathway associated with obesity, that may be predicted by KEGG annotation based on shotgun analysis.116. This evidence concerns the gene MTOR and obesity due to melanocortin 4 receptor deficiency.