NLRP3 and cryopyrin-associated periodic syndrome: To evaluate ASO-Δ2 and NLRP3Δ2 in an in vitro model directly relevant to clinical, pathological NLRP3 activation, we treated human monocyte-derived macrophages (hMDMs) from individuals with CAPS harboring an NLRP3 p.L353P mutation with the ASO.