To study whether polyGR+ aggregates are associated with known AD-risk loci, we next performed genotype experiments for nine known AD-associated single nucleotide polymorphisms (SNPs), including rs429358 (APOE4) and rs7412 (APOE2), rs744373 (BIN1), rs5848 (GRN), rs3851179 (PICALM), rs11218343 (SORL1), rs3173615 (TMEM106B), rs115550680 (ABCA7) and rs75932628 (TREM2) (summarized in Table 2 and Table S1). This evidence concerns the gene PICALM and Alzheimer disease.