In Tac1‐Ai9 mice, quantitative analyses revealed that 16.39% ± 6.78% of Tac1‐positive neurons showed c‐FOS/Tac1 double‐labeling following His‐induced itch (Figure 3D, Table 4), compared to 34.46% ± 5.18% in CQ‐induced acute itch models (Figure 3C, Table 5). Here, FOS is linked to Pruritus.