Upon double labelling and confocal immunofluorescent microscopy high levels of colocalization were observed between mHTT-IR and Ub-IR (Fig. 1E) or p62-IR and Ub-IR (Fig. 1F), implying colocalization of the three proteins within the inclusions, a pattern replicated in our study in the brain of a knock-in HD mouse model Q175 (53). This evidence concerns the gene INSR and Huntington disease.