We subsequently assessed the status of the ALP in HD brains, compared to control brains, with immunohistochemistry (IHC) of CTSD, which captures the status of both LY and AL collectively as CTSD is a lysosomal protease existing in both organelles and can serve as a marker for the ALP (AL can be distinguished from LY by residual presence of LC3 or another autophagy-specific substrate). The gene discussed is CTSD; the disease is Huntington disease.