To assess whether iTregs generated in the absence or inhibition of STAT6 remain stable under in vivo inflammatory conditions, we intravenously transferred FACS-sorted control iTregs, AS-iTregs, or STAT6−/− iTregs (3 × 105 cells) into mice during AOM/DSS-induced colitis (Fig. 4A). Here, STAT6 is linked to colitis.