To analyze the role of CERS6 in ESCC, the protein levels of CERS6 were measured in seven ESCC cell lines (KYSE30, KYSE70, KYSE140, KYSE150, KYSE410, KYSE450, KYSE510) and an immortalized esophageal cell line (SHEE) using Western blot. The gene discussed is CERS6; the disease is esophageal squamous cell carcinoma.