CD8A and non-small cell lung carcinoma: To identify master regulon(s) that may contribute to CD8+ differentiation along the cytotoxic effector trajectory (CX3CR1+Tems→Teff cells) in NSCLC tumors, we applied metaVIPER-based scRNA-seq analysis to construct GRNs of the cytotoxic effector branch (CX3CR1+Tems→Teff cells), the dysfunctional (TLdys) branch, and the exhausted (Texh) branch (Fig. 3a).