EGR4 and neoplasm: However, analysis of EGR4 expression in GC patients through TCGA data sets and paired para-cancerous and tumor tissues showed that it was upregulated in GC samples (Fig. 2B, C), and dual immunofluorescence staining also demonstrated that PanCK+ EGR4+ expression was higher in the metastatic lymph nodes than that in the primary tumors (Fig. 2D).