Our analyses revealed that DKK3-OE substantially exacerbated AD pathology: There was a 123.0% increase in the number of Aβ plaques (Aβ+), a 48.4% increase in microgliosis (IBA1+ area), and a 28.9% increase in astrogliosis (GFAP+ area) (Fig. 6, G to L). This evidence concerns the gene AIF1 and Alzheimer disease.