In the case of MASH samples, important parameters include the evaluation of basic hepatic features (albumin and urea production, expression of CYP3A4, CYP2D6, UGT1A1, ABCB11 and HNF4A), cellular composition of spheroids including cellular subtypes by immunostaining or FACS, insulin sensitivity by Western blot for phosphorylated AKT, apolipoprotein secretion, glucose uptake, steatosis as measured by imaging or quantification using the Adipored assay, inflammatory markers (e.g., IL6, IL8, TNFα) quantified by ELISA and fibrosis by staining for collagen 1A1. Here, TNF is linked to steatosis.