Normality of ΔΔCt values was assessed using the Shapiro–Wilk test (p > 0.05 for both genes); paired two-tailed t-tests were then applied to compare tumor versus matched normal tissues, yielding p = 0.003 for SPON2 and p = 0.02 for MSMB (Supplementary Table 3). This evidence concerns the gene SPON2 and neoplasm.