CCK-8 assay detected enhanced proliferative activity in the NAP1L1 high-expression group during the late culture period (72 hours) (p<0.0001, Figure 13B), and colony formation ability was also significantly enhanced (p<0.0001, Figure 13C), indicating that NAP1L1 can effectively promote HCC cell proliferative potential (Figure 13B). Here, NAP1L1 is linked to hepatocellular carcinoma.