ACTA2 and cancer: Six primary cell types were successfully delineated (Figures 2B, C), encompassing a total of 44,461 cells: (1) cancer cells expressing ALB, SERPINA1, and GPC3; (2) macrophages with high expression of FOLR2, AIF1, and CD68; (3) endothelial cells specifically expressing AQP1, TIE1, VWF, EDNRB, and CCL14; (4) fibroblasts enriched with ACTA2, COL1A1, DCN, and COL1A2; (5) hepatocytes with high expression of ALB and SERPINA1; and (6) NKT cells specifically expressing NKG7, GNLY, and CCL5.