Following 72 h, enhancement of tumor formation was appraised in human MSCs (hAMSCs)-treated HT-29 cells by EGFR/c-Src/IRSp53/p-AKT/p-Stat3 signal network employing qRT-PCR, besides the Western blot technique, as well as the MTT and hanging drop assays. This evidence concerns the gene EGFR and neoplasm.