Acidosis inhibits the activity of BPGM, thereby reducing levels of 2,3-BPG, while the increased lactate/NADH ratio suppresses glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity, leading to reduced formation of 1,3-BPG and consequently decreased 2,3-BPG production (51). Here, GAPDH is linked to Acidosis.