These splicing changes were validated by performing RT-PCR analysis of both CMTR2-knockout cell lines and the NCI-H1373 and NCI-H1915 lung cancer cell lines, which harbor truncating CMTR2 mutations (Fig. 4e–h, Supplementary Fig. 11a–c, and Supplementary Table 5). The gene discussed is CMTR2; the disease is lung carcinoma.